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Zinc
Code: 816
Specimens:
1.5mL serum (SST)
Minimum:
1mL serum (SST)
Storage:
Refrigerate
Availability:
Results available in 1-2 weeks
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Toxoplasmosis Antibody, IgG & IgM, Canine/Feline
Code: 391
Specimens:
1mL serum (SST)
Minimum:
0.5mL serum (SST)
Storage:
Refrigerate for up to 4 days; Freeze for longer storage.
Method:
IFA
Availability:
Results available in 3-7 days
Information:
See 'Procedures & Specimen Requirements' Page.
Interpretation:
IgG titer and IgM titer given individually. Approximately 30% of dogs and cats in the US have antibodies to T. gondii. A positive titer indicates exposure, but does not confirm infection nor clinical diagnosis. Tentative diagnosis requires demonstration of a high IgM titer (>256) or a fourfold (or greater) increase in IgG titer in 23 weeks.
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TSH
Code: 331
Specimens:
1mL serum (SST)
Minimum:
0.5mL serum (SST)
Storage:
Refrigerate for up to 4 days
Method:
Chemiluminescence
Availability:
Results available daily
Information:
See 'Procedures & Specimen Requirements' Page.
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Urine Protein/Creatinine Ratio
Code: 396
Specimens:
1.5mL urine (RTT/WTT)
Minimum:
1mL urine (RTT /WTT)
Storage:
Refrigerate for up to 4 days
Availability:
Results available daily
Information:
See 'Procedures & Specimen Requirements' Page.
Submission Requirements:
1mL urine in WTT (no additives)
Interferences:
Hematuria, pyuria
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Von Willebrand Factor
Code: 814
Specimens:
1mL citrated (BTT) plasma or EDTA plasma
Storage:
Freeze
Method:
ELISA
Availability:
Results available in 2-3 days
Information:
(See test procedure section)
Submission Requirements:
One plastic citrated blood tube (BTT) or EDTA plasma (EDTA) must be filled as much as vacuum will allow. Invert several times to mix anticoagulant and blood, then centrifuge immediately. Separate plasma from cells. Use plastic pipette to transfer plasma to plain plastic tube and freeze. Label tube “citrated plasma” along with client’s last name.
Interferences:
Hemoylsis, inclusion of RBC’s in plasma, or clotted specimen
Interpretation:
vWF AG= Von Willebrand Factor antigen measured in an ELISA
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Progesterone, Canine
Code: 379
Specimens:
1.5mL serum (WTT) DO NOT use serum separator tubes
Minimum:
1mL serum (WTT)
Storage:
Refrigerate for up to 4 days
Method:
Chemiluminescence
Availability:
Results available within 24-36 hours
Information:
See 'Procedures & Specimen Requirements' Page.
Submission Requirements:
1.5mL serum (WTT) DO NOT USE SST
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Prothrombin Time (PT)
Code: 381
Specimens:
1mL citrated plasma (BTT). All blue top tubes must be filled to capacity for accurate analysis.
Minimum:
0.5mL citrated plasma (BTT)
Storage:
Centrifuge sample. Aliquot the plasma into a separate tube and keep refrigerated if specimen will arrive in the laboratory within 24 hours. Otherwise, freeze plasma within 1 hour of collection and submit frozen plasma to the laboratory.
Method:
Mechanical clot detection (Automated)
Availability:
Results available daily
Information:
The blood to anticoagulant ratio is critical for accurate test results. If an inadequate blood sample is taken, results will be falsely prolonged. Clearly label tube as citrated plasma.
Submission Requirements:
1mL citrated plasma (BTT). Tube must be filled as much as vacuum will allow. Invert several times to mix anticoagulant and blood and then centrifuge immediately. Separate plasma from cells. Transfer plasma to WTT, not another BTT. Label tube “citrated plasma” along with client’s last name.
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PTT (Activated Partial Thromboplastin Time)
Code: 383
Specimens:
1mL citrated plasma (BTT). All blue top tubes must be filled to capacity for accurate analysis.
Minimum:
0.3mL citrated plasma (BTT)
Storage:
Centrifuge sample. Aliquot the plasma into a separate tube and keep refrigerated if specimen will arrive in the laboratory within 24 hours. Otherwise, freeze plasma within 1 hour of collection and submit frozen plasma to the laboratory.
Method:
Mechanical clot detection/Amelung
Availability:
Results available daily
Information:
The blood to anticoagulant ratio is critical for accurate test results. If an inadequate blood sample is taken, results will be falsely prolonged. Clearly label tube as citrated plasma.
Submission Requirements:
Citrated plasma (BTT). Tube must be filled as much as vacuum will allow. Invert several times to mix anticoagulant and blood and then centrifuge immediately. Separate plasma from cells. Transfer plasma to WTT, not another BTT. Label tube “citrated plasma” along with client’s last name.
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Rheumatoid Factor
Code: 504
Specimens:
1mL serum (SST)
Storage:
Refrigerate for up to 4 days
Method:
Latex Agglutination
Availability:
Results available in 23 days
Information:
See 'Procedures & Specimen Requirements' Page.
Submission Requirements:
1mL serum (SST)
Interferences:
Osteoarthritis, fibrositis, polyarteritis nodosa, EDTA
Interpretation:
Positive or Negative
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Stone Analysis
Code: 394
Specimens:
Air-dried specimen in a clean, dry container.
Storage:
Room Temperature
Method:
Quantitative
Availability:
Results available in 1-2 weeks
Information:
See 'Procedures & Specimen Requirements' Page.
Submission Requirements:
Calculi in a clean, dry container
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T4, Total
Code: 387
Specimens:
0.5mL serum (SST)
Storage:
Refrigerate for up to 4 days
Method:
Chemiluminescence
Availability:
Results available daily
Information:
See 'Procedures & Specimen Requirements' Page.
Submission Requirements:
0.5mL serum (SST)
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Testosterone
Code: 818
Specimens:
1mL serum (SST)
Storage:
Refrigerate for up to 4 days
Method:
RIA
Availability:
Results available in 3-8 days
Information:
See 'Procedures & Specimen Requirements' Page.
Submission Requirements:
1mL serum (SST)
Canine:
Male, intact 17 ng/mL
Feline:
Male, intact 16 ng/ml
Interferences:
EDTA
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Phenobarbital
Code: 374
Specimens:
1mL of serum (WTT). DO NOT use a serum separator tube.
Minimum:
0.5mL of serum (WTT)
Storage:
Refrigerate for up to 4 days
Method:
Chemiluminescence
Availability:
Results available daily
Information:
See 'Procedures & Specimen Requirements' Page.
Submission Requirements:
1mL serum (WTT), do not use SST
Subtherapeutic Level:
<15 ug/ml Therapeutic level 1540 ug/ml Possible toxic level >40 ug/ml The biological halflife of Phenobarbital in the dog ranges from 37–75 hours. The steady state is generally only achieved after 23 weeks of drug therapy. Due to patient variability (sensitivity and tolerance) serum Phenobarbital values must be evaluated in conjunction with the patient’s clinical signs. * If patient is on both potassium bromide and Phenobarbital, we recommend monitoring serum levels of both drugs.
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Iron & Iron Total Binding Capacity, % Saturation
Code: 503
Specimens:
2mL serum (SST) or heparinized plasma (GTT)
Minimum:
1mL serum (SST) or heparinized plasma (GTT)
Storage:
Frozen; Refrigerate for up to 4 days
Method:
Spectrophotometric
Availability:
Results available in 4-7 days
Information:
See 'Procedures & Specimen Requirements' Page.
Submission Requirements:
2mL serum (SST) ; Centrifuge within two hours of blood collection, remove serum from blood tube, and then place into plastic tube (WTT) for freezing.
Interferences:
Hemolysis, lipemia
Interpretation:
This panel is commonly used for the diagnosis of iron deficiency or anemia of inflammatory disease. Either of these syndromes can demonstrate serum iron levels that range from normal to low. In cases of iron deficiency, TIBC will often be high to normal, and serum ferritin will be low. Patients affected by anemia of inflammatory disease will have a normal to low TIBC .
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Lead, Blood
Code: 367
Specimens:
1mL whole blood (EDTA)
Minimum:
0.5mL whole blood (EDTA)
Storage:
Refrigerate
Method:
Atomic absorption
Availability:
Results available in 3-5 days
Information:
See 'Procedures & Specimen Requirements' Page.
Submission Requirements:
1mL whole blood (EDTA) or heparinized whole blood (GTT) *DO NOT submit serum for this test.
Interferences:
Incorrect tube
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Feline Leukemia Virus Antigen IFA
Code: 351
Storage:
Refrigerate for up to 4 days, store slides at room temperature.
Method:
IFA
Availability:
Results available in 7-10 days
Information:
See 'Procedures & Specimen Requirements' Page.
Submission Requirements:
2 air-dried UNSTAINED bone marrow or peripheral blood smears or 1mL whole blood (EDTA)
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Fructosamine
Code: 354
Specimens:
1mL serum (SST)
Minimum:
0.5mL serum (SST)
Storage:
Refrigerate for up to 4 days
Method:
Colormetric/ Chemistry analyzer
Availability:
Results available daily
Information:
See 'Procedures & Specimen Requirements' Page.
Submission Requirements:
1mL serum or plasma (SST or GTT); nonhemolyzed, fasted sample
Interpretation:
This test reflects glycemic status over the previous 23 weeks.
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Fungal Culture
Code: 334
Storage:
Refrigerate for up to 4 days
Method:
Specimens plated on selective media for the isolation of fungi
Availability:
Set up daily. Cultures held 4 weeks before final reporting
Information:
See 'Procedures & Specimen Requirements' Page.
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Fungal Serology Panel (Histoplasma, Blastomyces, Coccidioides, Aspergillus)
Code: 121
Specimens:
2mL serum (SST)
Storage:
Refrigerate for up to 4 days
Method:
AGID
Availability:
Results available in 5-7 days
Information:
See 'Procedures & Specimen Requirements' Page.
Submission Requirements:
3mL serum (SST)
Interferences:
Anticoagulants (EDTA or citrate) or preservatives
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Distemper and Parvo Titer (Canine)
Code: 8661
Specimens:
1mL serum (SST) or plasma (EDTA)
Minimum:
0.5mL serum (SST) or plasma (EDTA)
Storage:
Refrigerate for up to 4 days
Method:
ELISA
Availability:
Results available daily
Information:
See 'Procedures & Specimen Requirements' Page.
Submission Requirements:
1mL serum (SST)
Interferences:
Hemolysis
Interpretation:
A positive distemper ELISA result correlates with an SN antibody titer ≥ 116, indicating probable protective immunity. A positive parvovirus ELISA result correlates with an HI antibody titer ≥180, indicating probable protective immunity. A negative result indicates a low antibody level, and a booster vaccination should be considered.
Includes:
Distemper and Parvovirus antibody
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Ethylene Glycol
Code: 345
Specimens:
2-3mL serum (SST), 2-3mL EDTA plasma or 2mL ascites
Minimum:
2mL serum (SST)
Storage:
Refrigerate
Method:
Colormetric / precipitation extractions
Availability:
Results available daily
Information:
See 'Procedures & Specimen Requirements' Page.
Interpretation:
Positive or Negative
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Fecal Occult Blood
Code: 347
Specimens:
1 tablespoon feces in sterile fecal collection tube/WTT
Storage:
Refrigerate
Method:
Guaiac test
Availability:
Results available daily
Information:
(See test procedure section)
Submission Requirements:
1 tablespoon fresh feces
Suggested Protocol:
Avoid red meat, (liver, beef, and lamb) and their byproducts for 23 days. While remaining on this special diet, collect a stool sample once a day for 3 days. Pool the samples together and send it in on the third day.
Interferences:
Aspirin, corticosteroids, iron supplements, dietary blood, horseradish, red meat diet
Interpretation:
Positive or Negative
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Coombs
Code: 324
Specimens:
1mL whole blood (EDTA)
Minimum:
0.5mL whole blood (EDTA)
Storage:
Refrigerate
Method:
Hemagglutination
Availability:
Results available in 3-5 days
Information:
Specimen must be analyzed within 24-36 hours of collection. Steroid therapy may cause false negative results.
Submission Requirements:
1mL whole blood (EDTA)
Interferences:
Steroid therapy, severe autoagglutination
Interpretation:
Positive or Negative with degree of agglutination
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Cryptococcus Antigen
Code: 501
Specimens:
1mL of CSF or serum (SST)
Minimum:
0.5mL of CSF or serum (SST)
Storage:
Refrigerate
Method:
Latex Agglutination
Availability:
Results available in 2-5 days
Information:
See 'Procedures & Specimen Requirements' Page.
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Dexamethasone Suppression, High Dose (Baseline, 4-hour, & 8-hour) (canine)
Code: 300
Specimens:
0.5mL serum (SST) each (Heparinized plasma also acceptable)
Minimum:
0.3mL serum (SST)
Storage:
Refrigerate. Freeze if stored more than 3 days
Method:
Chemiluminescence
Availability:
Results available daily
Information:
Sampling time Baseline, 4hour, and 8hour post
Submission Requirements:
0.5mL serum (SST) per specimen
Dosage:
0.1mg/kg IV
Baseline Cortisol:
15ug/dL
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Dexamethasone Suppression, Low Dose (Baseline, 4-hour, & 8-hour) (canine)
Code: 127
Specimens:
0.5mL serum (SST) each (Heparinized plasma also acceptable)
Minimum:
0.3mL serum (SST)
Storage:
Refrigerate. Freeze if stored more than 3 days
Method:
Chemiluminescence
Availability:
Results available daily
Information:
Sampling time Baseline, 4hour, and 8hour post
Submission Requirements:
0.5mL serum (SST) per specimen
Feline:
Dexamethasone suppression assays are not as easily interpreted in the cat as in the dog. Cortisol values of >1.5 ug/dL in either of the two post dexamethasone samples suggest hyperadrenocorticism.
Dosage:
0.01mg/kg IV
Baseline Cortisol:
1.05.0 ug/dL
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CALP/ALP
Code: 431
Specimens:
0.5mL serum (SST)
Minimum:
0.3mL serum (SST)
Storage:
Refrigerate
Method:
Automated chemistry analyzer
Availability:
Results available daily
Information:
See 'Procedures & Specimen Requirements' Page.
Submission Requirements:
0.5mL serum (SST)
Interpretation:
The corticosteroid induced isoenzyme of alkaline phosphate (CALP) is a unique enzyme found only in dogs. Normal dogs have little or none of this enzyme. Its presence in serum is often observed in dogs with Cushing’s disease, iatrogenic Cushing’s syndrome and in some older dogs with chronic disease. In dogs with greater than 30 U/L CALP activities, an examination for clinical signs of Cushing’s disease as well as an investigation of history of prior treatment with glucocorticoids should be made. Additional diagnostic testing for Cushing’s disease may be warranted. The sensitivity for CALP in the diagnosis of Cushing’s disease is nearly 100% but the specificity is in the order of 85%. In other words, Cushing’s disease is very unlikely if the CALP activity is not increased.
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Bile Acids, Pre & Post
Code: 119
Specimens:
0.5mL serum (SST) pre-prandial and 0.5 mL serum (SST) post prandial from fasting patient (10-12 hours)
Storage:
Refrigerate
Method:
Enzymatic/Automated chemistry analyzer
Availability:
Results available daily
Information:
See 'Procedures & Specimen Requirements' Page.
Submission Requirements:
0.5mL serum (SST) per specimen
Suggested Protocol:
1) Fast the animal approximately 12 hours and obtain a fasting (preprandial) serum specimen of at least 1mL. Label the tube Fasting or Preprandial. 2) Ideally, feed the animal a highfat meal in order to stimulate gallbladder contractions. Minimum amount of food is 2 teaspooons for small patients (<10lb) and 2 tablespoons for large patients.
Interferences:
Hemolysis, lipemia, and icterus
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Blood Typing, Canine
Code: 310
Specimens:
3mL whole blood (EDTA)
Minimum:
1.5mL whole blood (EDTA)
Storage:
Refrigerate
Availability:
Results available daily
Submission Requirements:
1mL whole blood (EDTA)
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Bromide (KBR)
Code: 378
Specimens:
2mL serum (WTT). DO NOT use a serum separator tube
Minimum:
0.4mL serum (WTT)
Storage:
Refrigerate
Method:
Automated chemistry analyzer
Availability:
Results available daily
Information:
(See test procedure section)
Submission Requirements:
1mL serum (WTT); Do not submit in SST
Interferences:
Hemolysis, icterus, and SST gel
Nonloading Dose Treatment Protocol:
Measure bromide concentration one month into treatment and again at 2 to 3 months (steadystate concentration). 1 month bromide concentration approximates ½ of future steady state concentrations; Therefore, dose may be adjusted proactively.
Loading Dose Treatment Protocol:
1 month into treatment, and again in 23 months. If postloading and one month concentrations vary more than 10% from each other, maintenance dose can be modified accordingly.
Therapeutic Range:
Canine: 12.mg/mL (100200 mg/dl) in combination with Phenobarbital or 2.5 – 3.0 mg/mL (250300mg/dl) as sole anticonvulsant (without Phenobarbital) * Steadystate drug concentrations are expected in 34 months. Due to patient variability, therapeutic drug concentrations should be interpreted in combination with patient’s clinical signs. (Also see Phenobarbital)
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Alkaline Phosphatase, Corticosteroid-induced (CALP), Canine
Code: 316
Specimens:
0.5mL serum (SST)
Minimum:
0.3mL serum (SST)
Storage:
Refrigerate
Method:
Automated chemistry analyzer
Availability:
Results available daily
Interferences:
Lipemia and hemolysis may alter serum chemistry results
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Aspergillus AGID
Code: 500
Specimens:
2mL serum (SST)
Minimum:
1mL serum (SST)
Storage:
Refrigerate
Method:
Immunodiffusion
Availability:
Results available in 3-5 days
Submission Requirements:
1mL serum
Interferences:
Marked hemolysis, lipemia
Interpretation:
Positive or Negative
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Baermann Fecal (lungworm)
Code: 808
Specimens:
2-5g of fresh feces
Storage:
Refrigerate
Availability:
Results available in 3-5 days
Information:
Do not allow specimen to touch ground or be exposed to flies.
Submission Requirements:
2-5g fresh feces in sterile container; do not use formalin
Interpretation:
No parasites seen or parasites identified
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ACTH Stimulation, Canine (Baseline & 1-hour)
Code: 126
Specimens:
0.5mL serum (SST) each. Centrifuge and remove serum from cells within 30 minutes of collection. Write actual time drawn on the tube.
Minimum:
0.2mL serum (SST) each
Storage:
Refrigerate. Freeze if stored more than 3 days.
Method:
Chemiluminescence
Availability:
Results available daily
Procedure Sampling Time:
Pre and 1-hour post Drug Synthetic ACTH Dosage 0.25mg IV or 5ug/kg. (See test procedure section)
Reference Range:
Baseline cortisol 1.0-5.0ug/dL. ACTH post stimulation 10-20ug/dL. Values above 20ug/dL in the post ACTH cortisol assay are suggestive of hyperadrenocorticism. However, these results are not specific for hyperadrenocorticism. Dogs with a variety of disease processes may have ACTH stimulation assay results in the same range as dogs with hyperadrenocorticism. The diagnosis of hyperadrenocorticism must be further substantiated by appropriate clinical signs and other laboratory assay results. Baseline cortisol results that are low or below limit of detection which fail to increase with the administration of ACTH are consistent with hypoadrenocorticism.
Submission Requirements:
0.5mL serum per specimen (do not submit EDTA plasma)
Suggested Protocol:
Fast the patient approximately 12 hrs (preferred, not essential) and collect 3mL of blood. Centrifuge and separate at least 0.5mL of serum for a resting cortisol.
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CBC, Chem. 7
Code: 157
Specimens:
SST, EDTA
Storage:
Refrigerate
Availability:
Results available daily
No results were found.