PTT (Activated Partial Thromboplastin Time)
1mL citrated plasma (BTT). All blue top tubes must be filled to capacity for accurate analysis.
0.3mL citrated plasma (BTT)
Centrifuge sample. Aliquot the plasma into a separate tube and keep refrigerated if specimen will arrive in the laboratory within 24 hours. Otherwise, freeze plasma within 1 hour of collection and submit frozen plasma to the laboratory.
Mechanical clot detection/Amelung
Results available daily
The blood to anticoagulant ratio is critical for accurate test results. If an inadequate blood sample is taken, results will be falsely prolonged. Clearly label tube as citrated plasma.
Citrated plasma (BTT). Tube must be filled as much as vacuum will allow. Invert several times to mix anticoagulant and blood and then centrifuge immediately. Separate plasma from cells. Transfer plasma to WTT, not another BTT. Label tube “citrated plasma” along with client’s last name.
Incorrect ratio of citrate to whole blood, clotted specimen, non-citrate anticoagulant.